Regulatory

Part:BBa_K2148003:Design

Designed by: Chern Juin Ong   Group: iGEM16_Cambridge-JIC   (2016-10-09)


atpA promoter/5UTR


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 543
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Sequence flanked with the necessary Phytobrick fusion sites to conform with the Phytobrick Standard

One point mutation was introduced to conform to Phytobrick Standard to remove illegal sites at base pair number 10.

Source

Chlamydomonas reinhardtii

References